Cross Reactivity of Dihydrodigoxin with a Commercial Digoxin
نویسندگان
چکیده
1086 CLINICAL CHEMISTRY, Vol. 24, No. 6, 1978 ally, the normal introduction time of 2 s leads to the good precision and recovery as found with the use of larger sample volumes, as discussed in the original letter. Corning has completed a rewording of their instruction manual to clarify the statement “slowly inject the sample until the ‘Sample in Place’ indicator lights,” so that it is obvious to the user that optimum results are obtained with introduction times of between 2 and 3
منابع مشابه
Variability among commercially available digoxin radioimmunoassay kits in cross reactivity to dihydrodigoxin.
We evaluated four commercially available 125I-digoxin radioimmunoassay kits with regard to their ability to cross react with the digoxin metabolite dehydrodigoxin. We prepared dihydrodigoxin serum samples in digoxin-free serum over the concentration range 0.4 to 5.0 microgram/liter and assayed them with each kit according to the manufacturer's instructions. The metabolite was able to displace t...
متن کاملCross reactivity of dihydrodigoxin with a commercial digoxin kit.
1086 CLINICAL CHEMISTRY, Vol. 24, No. 6, 1978 ally, the normal introduction time of 2 s leads to the good precision and recovery as found with the use of larger sample volumes, as discussed in the original letter. Corning has completed a rewording of their instruction manual to clarify the statement “slowly inject the sample until the ‘Sample in Place’ indicator lights,” so that it is obvious t...
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Our objective was to identify commercially available digoxin immunoassays whose cross-reactivity with digoxin metabolites paralleled the pharmacological activity of the metabolites. We measured the immunoreactivity of digoxigenin bis- and monodigitoxosides, digoxigenin, and dihydrodigoxin in four immunoassays and compared the immunoactivities with pharmacological activities from studies involvi...
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Digoxin-like immunoreactive factor (DLIF) from adrenal glands is an endogenous ligand structurally related to the plant-derived cardiac glycoside digoxin. Cardiac glycosides regulate the activity of the sodium pump and thus play key roles in disease processes involving regulation of ion transport. We now report the discovery of an endogenous dihydro-DLIF analogous to dihydrodigoxin. We used HPL...
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the treatment of congestive heart failure and atrial fibrillation. It is important to measure digoxin in serum from digitalized patients because the therapeutic range of serum digoxin concentration is narrow. Immunoassays are routinely used for therapeutic drug monitoring (TDM) of digoxin. The antibody with cross-reactivity to metabolites of digoxin (digoxigenin bis and monodigitoxosides, digox...
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